For most organizational members, the workplace presents a coexistence of both positive and negative emotional experiences. However, through the compassion of colleagues, the workplace can transform into a site for high-quality relationships and human healing. From this perspective, this study empirically investigates the antecedents and consequences of compassion at work. Specifically, we propose that members' perception of their work-life balance functions as a psychological resource and an antecedent of their compassion at work. We also examine the mediating role of compassion at work in the relationship between members' perception of their work-life balance and organizational commitment. Additionally, we verify the moderating role of members' emotional intelligence in the activation of compassion at work. We collected survey data from 339 employees at a manufacturing company. Our major findings are as follows: First, a positive relationship exists between organizational members' perception of their work-life balance and compassion at work. Second, we observed the mediating role of compassion at work in the relationship between members' perception of work-life balance and their organizational commitment. Furthermore, an organizational member's emotional intelligence moderated the positive relationship between the perception of work-life balance and compassion at work, and it also moderated the mediating effects of compassion at work. Based on these findings, we discuss the managerial implications of this research and future research directions.